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Microscope

显微镜

Company: Leica Microsystems
Catalog#: Leica M165 C
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MiniSOG-mediated Photoablation in Caenorhabdtis elegans
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2013-01-05
[Abstract]  This protocol describes a method for light-inducible cell ablation in live worms. miniSOG (mini Singlet Oxygen Generator) generates singlet oxygen upon blue light illumination (Shu et al., 2011). Mitochondrially membrane targeted miniSOG (the first 55 a. a. of C. e. tomm-20 fused at the N’-terminus of miniSOG, termed as mito-miniSOG in the following) is transgenically expressed in specific cells/tissues (Qi et al., 2012). Groups of transgenic animals are illuminated under open field fluorescence light on a compound microscope or LED light setup for photo-ablation. [摘要]  该协议描述了在活蠕虫中的光诱导细胞消融的方法。 miniSOG(微型单态氧发生器)在蓝光照射时产生单线态氧(Shu等人,2011)。 线粒体膜靶向的miniSOG(在miniSOG的N'末端融合的Cys的第一个55aa,在下文中称为mito-miniSOG)在特定细胞/组织中转基因表达, 组织(Qi等人,2012)。 在复合显微镜或用于光消融的LED光设置下在开放场荧光下照射转基因动物组。

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