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Author:
Date:
2015-04-20
[Abstract] During combined nitrogen step-down, filaments of cyanobacterium Anabaena sp. PCC 7120 differentiate about 5-10% of vegetative photosynthetic cells into heterocysts, the specialized cells for N2 fixation (Walk, 1996). Heterocysts have a thick cell wall reducing permeation of O2 and consist of two additional layers composed of glycolipids and polysaccharides. The difference in structure and composition of the cell wall between heterocysts and vegetative cells allows separation and isolation of heterocyst. Heterocysts isolated by this protocol can be subjected to protein analysis and activity measurements, which do not require strict anaerobic conditions.
[摘要] 在组合氮降压期间，蓝细菌的鱼腥藻属 PCC 7120将约5-10％的营养光合细胞分化成异种细胞，这是用于N sub2固定的特化细胞（Walk，1996）。 杂合体具有厚的细胞壁，减少O 2的渗透，并且由两个由糖脂和多糖组成的附加层组成。 杂合细胞和营养细胞之间的细胞壁的结构和组成的差异允许异种细胞的分离和分离。 通过该方案分离的杂合体可以进行蛋白质分析和活性测量，其不需要严格的厌氧条件。
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