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Author:
Date:
2015-04-05
[Abstract] For monitoring the cellular pH in plants, engineered green fluorescent protein (GFP) has been used to indicate cellular pH. Pt-GFP is a pH-reporter protein which has been used ratiometrically in the double excitation mode, according to the fluorescence properties described for Pt-GFP (Schulte et al., 2006), and depending on our previous experiments, we present here a little and efficient protocol for monitoring the cytosolic pH value. In this protocol, root hair cellular pH was monitored in the PtGFP reporter lines. Cellular pH can be obtained according to the calibration curve which was performed in situ by using a series pH buffer.
[摘要] 为了监测植物中的细胞pH，使用工程绿色荧光蛋白（GFP）来指示细胞pH。 根据Pt-GFP（Schulte等人，2006）所述的荧光性质，Pt-GFP是pH报道蛋白，其已经在双激发模式中按比例计量使用，并且取决于我们 以前的实验，我们在这里提出一个有效的协议，用于监测胞浆pH值。 在该协议中，在PtGFP报告系中监测根毛细胞pH。 可以根据通过使用系列pH缓冲液原位进行的校准曲线获得细胞pH。
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