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Company: NISSUI PHARMACEUTICAL

Catalog#: 05835

Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay

Pirjo Pärnänen, Timo Sorsa, Taina Tervahartiala and Pirjo Nikula-Ijäs

Published online: 2024-03-20

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Purification of 70S Ribosomes from Bacillus subtilis

Author:

Shota Suzuki, Genki Akanuma and Fujio Kawamura,

Date:

2015-04-05

[Abstract] The eubacterial ribosome (70S) is a macromolecular complex that is composed of a small (30S) subunit and a large (50S) subunit. The small subunit comprises the 16S ribosomal RNA (rRNA) and more than 20 ribosomal proteins (r-proteins), whereas the large subunit comprises the 23S and 5S rRNAs and more than 30 r-proteins. Bacillus subtilis (B. subtilis) has 57 r-ribosomal protein genes and three rRNAs (16S, 23S and 5S rRNAs). Among them, we identified 21 r-proteins of the small subunit and 31 r-proteins of the large subunit in B. subtilis (Nanamiya et al., 2004). The functions and roles of individual components of the ribosome have not yet been completely clarified. Herein we describe in detail an ultracentrifugation-based protocol for the preparation of ... [摘要] 真细菌核糖体（70S）是由小（30S）亚基和大（50S）亚基组成的大分子复合物。小亚基包含16S核糖体RNA（rRNA）和超过20个核糖体蛋白（r-蛋白），而大亚基包含23S和5S rRNA和超过30个r蛋白。枯草芽孢杆菌（<枯草芽孢杆菌）具有57个核糖体蛋白基因和三个rrna（16s，23s和5s rrna）。其中，我们确定21 r蛋白的小亚基和31 r蛋白的大亚基在b。枯草芽孢杆菌（nanamiya等人，2004）。核糖体的各个组分的功能和作用尚未完全澄清。在本文中，我们详细描述了从b的指数生长细胞制备70s核糖体的基于超速离心的方案。枯草芽孢杆菌。 rrna）。="" 其中，我们确定21="" r蛋白的小亚基和31="" r蛋白的大亚基在b。="" 枯草芽孢杆菌（nanamiya等人，2004）。="" 核糖体的各个组分的功能和作用尚未完全澄清。="" 在本文中，我们详细描述了从b的指数生长细胞制备70s核糖体的基于超速离心的方案。="" 枯草芽孢杆菌。=""> ...

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