| Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
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Author:
Date:
2012-02-05
[Abstract] Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then differentiated to bone marrow-derived macrophages in approximately 10-day culture using L929-conditioned medium.
[摘要] 骨髓来源的巨噬细胞是一种可以从哺乳动物骨髓中分离的白细胞。 在该方案中，描述了一种方法，其中从小鼠腿骨（股骨和胫骨）分离骨髓细胞，然后使用L929条件培养基在大约10天培养物中分化为骨髓来源的巨噬细胞。
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| siRNA Transfection of Mouse Bone Marrow-derived Macrophages
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Author:
Date:
2011-02-05
[Abstract] Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in BMM’phi’ cells. Two days after transfection with cells at 60-80% confluence, the knockdown efficiency can reach 90%.
[摘要] 短干扰RNA（siRNA）是一种双链RNA分子，通常为20-25bp长，参与RNA干扰现象。 siRNA转染在该方案中用于在BMM'phi'细胞中敲低靶基因表达。 在60-80％汇合下用细胞转染两天后，击倒效率可以达到90％。
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