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Carestream® Kodak® BioMax® XAR Film

Carestream ®柯达® BioMax ® XAR Film

Company: Sigma-Aldrich
Catalog#: F5763
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Protein Translation Study – Label Protein with S35 Methionine in Cells
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Date:
2012-11-05
[Abstract]  To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific antibody and separation on a polyacrylamide denaturing gel. [摘要]  为了遵循蛋白质合成,在mRNA翻译期间,细胞应与放射性氨基酸如[35S]甲硫氨酸一起温育。 然后,在用特异性抗体免疫沉淀并在聚丙烯酰胺变性凝胶上分离后,通过放射自显影鉴定新合成的蛋白质。

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