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Author:
Date:
2014-12-05
[Abstract] Multidrug resistance Acinetobacter baumannii (A. baumannii) (MDRAB) has emerged as a serious threat in hospitals in recent years. Currently, there are few antibiotics, including tigecycline, available to treat infections caused by MDRAB effectively. Both tigecycline-resistant and tigecycline-susceptible isogenic strains of MDRAB are valuable in understanding the mechanisms underlying tigecycline resistance. To get the isogenic strains in the laboratory, we describe a protocol for induction of tigecycline resistance in A. buamannii by serial passage to plates with tigecycline of different concentrations. The minimal inhibitory concentration of A. baumannii by tigecycline was determined according to the protocol “Minimal Inhibitory Concentration (MIC) ...
[摘要] 多药耐药近年来，在医院中，抗巨细胞鲍氏不动杆菌（HA）抗体（MDRAB）已经成为严重的威胁。 目前，几乎没有抗生素，包括替加环素，可有效治疗MDRAB引起的感染。 MDRAB的替加环素抗性和替加环素敏感的同基因菌株对于了解替加环素抗性的机制是有价值的。 为了在实验室中获得同基因菌株，我们描述了在A中诱导替加环素抗性的方案。 buamannii）通过连续进入具有不同浓度替加环素的平板。 A的最小抑制浓度。 根据方案"最小抑制浓度（MIC）测定法"确定替加环素的阿米巴 鲍氏不动杆菌" "（Lin等人，2014b）。
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