Falcon® 24 Well Multiwell Flat Bottom TC-Treated Cell Culture Plate, with Lid, Sterile
Company: BD
Catalog#: 353226
Product evaluation:
Secretion of Adipsin as an Assay to Measure Flux from the Endoplasmic Reticulum (ER)
Author:
Date:
2017-04-05
[Abstract]  In this protocol we describe a quantitative biochemical assay to assess the efficiency of endoplasmic reticulum (ER) to Golgi protein transport in adipocytes (Bruno et al., 2016). The assay takes advantage of the fact that adipocytes secrete various bioactive proteins, known as adipokines. As a measure of ER to Golgi flux we determine the rate of bulk secretion of the adipokine adipsin post washout of Brefeldin A (BFA) treatment using immunoblotting. Because BFA treatment results in an ...

Mouse CD8+ T Cell Migration in vitro and CXCR3 Internalization Assays
Author:
Date:
2017-03-20
[Abstract]  Chemokines are molecules that regulate the positioning of cells during homeostasis and inflammation. CXCL10 is an interferon-induced chemokine that attracts cells that express the chemokine receptor CXCR3 on their surface. CXCL10 expression is often induced upon inflammation and guides lymphocytes, such as T and NK cells, into the injured tissues. Notably, CXCL10 binding to CXCR3 induces receptor internalization and, therefore, low CXCR3 levels in cells positive for CXCR3 expression can be ...

Knock-in Blunt Ligation Utilizing CRISPR/Cas9
Author:
Date:
2017-03-05
[Abstract]  The incorporation of the CRISPR/Cas9 bacterial immune system into the genetic engineering toolbox has led to the development of several new methods for genome manipulation (Auer et al., 2014; Byrne et al., 2015). We took advantage of the ability of Cas9 to generate blunt-ended double-strand breaks (Jinek et al., 2012) to introduce exogenous DNA in a highly precise manner through the exploitation of non-homologous end-joining DNA repair machinery (Geisinger et al ...

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