| Expression, Purification and Enzymatic Assay of Plant Histone Deacetylases
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Author:
Date:
2016-04-05
[Abstract] Histone deacetylases (HDACs) catalyzing the removal of acetyl groups from lysine residues of histone and non-histone proteins play vital roles in regulation of gene transcription. In plants, HDACs can be grouped into three families, including RPD3-type, SIR2-type and plant specific HD2-type HDACs. Here we describe a method to determine plant HDAC enzymatic activity. This protocol includes expression, purification and enzymatic activity assay of recombinant plant HDACs expressed in Escherichia coli (E. coli) and Arabidopsis thaliana (A. thaliana).
[摘要] 组蛋白脱乙酰酶（HDAC）催化从组蛋白和非组蛋白蛋白的赖氨酸残基去除乙酰基在调节基因转录中起重要作用。 在植物中，HDAC可以分为三个家族，包括RPD3型，SIR2型和植物特异性HD2型HDAC。 在这里我们描述了一种确定植物HDAC酶活性的方法。 该方案包括在大肠杆菌（大肠杆菌）和拟南芥（）中表达的重组植物HDAC的表达，纯化和酶活性测定 >拟南芥）。
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| Karyotype Analysis
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Author:
Date:
2014-05-20
[Abstract] A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to be one of the hallmarks of tumor formation. Here we use the karyotype analysis to separate the chromosomes and observe the chromosomes in tumor cells with a microscope.
[摘要] 染色体是组织细胞中的DNA和蛋白质的结构。 它是含有编码和非编码序列的单片卷曲DNA。 人类细胞具有23对染色体，包括22对常染色体和一对性染色体，每个细胞总共46个染色体。 在肿瘤细胞中，染色体不稳定性已被认为是肿瘤形成的标志之一。 这里我们使用核型分析来分离染色体，并用显微镜观察肿瘤细胞中的染色体
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| Preparation of Primary Neurons from Rat Median Preoptic Nucleus (MnPO)
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Author:
Date:
2013-12-05
[Abstract] Studying cell physiology is really important to understand their function and to determine action mechanisms taking place in these cells. Using brain slices can be sometime difficult to directly access to cells like neurons. Even if it more steps are needed to get cultured cells, this type of preparation allow a better access to neurons and provide a good way to study their basal properties. Here we describe a protocol of a short term primary neurons culture from MnPO, allowing to kept neurons in good condition to perform electrophysiological recordings.
[摘要] 研究细胞生理学对于了解其功能和确定在这些细胞中发生的作用机制是非常重要的。 使用脑切片可能有时难以直接访问像神经元这样的细胞。 即使需要更多的步骤来培养细胞，这种类型的制剂可以更好地接近神经元，并提供研究其基础性质的好方法。 在这里，我们描述了一种来自MnPO的短期原代神经元培养的方案，允许神经元保持良好的状态进行电生理记录。
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