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Author:
Date:
2012-07-05
[Abstract] Many important regulatory proteins such as transcription factors are regulated through subcellular localization. Protein localization can be examined by fusing a GFP tag. However, GFP is relatively big in size, and potentially may affect correct protein localization. Several small tags have been developed, such as myc, HA or Flag. By using immunostain and fluorescence microscopy as described in this protocol, one can easily probe the regulation of a selected yeast protein with the application of the aforementioned small tags.
[摘要] 许多重要的调节蛋白如转录因子通过亚细胞定位进行调节。 蛋白质定位可以通过融合GFP标签来检查。 然而，GFP在尺寸上相对较大，并且潜在地可能影响正确的蛋白质定位。 已经开发了几个小标签，例如myc，HA或Flag。 通过使用如本方案中所述的免疫染色和荧光显微镜，可以通过应用上述小标签容易地探测所选择的酵母蛋白的调节。
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