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Author:
Date:
2013-12-05
[Abstract] Investigations of the activation processes involved in human monocytes and monocyte-derived macrophages and dendritic cells often required large numbers of cells that have not been possibly altered or activated by adherence to surfaces, by binding of antibodies to surface antigens during positive selection, or by release of activators by platelets or other non myeloid cells during isolation or co-culture. Human peripheral blood monocytes as well as lymphocytes from the same blood donor can be isolated by counterflow elutriation using a modification of the technique of Lionetti et al. (1980) as described previously (Bobak et al., 1986). From a unit of blood drawn into anticoagulant, 60-120 million monocytes can be obtained. These cells are not activated and have been ...
[摘要] 涉及人单核细胞和单核细胞衍生的巨噬细胞和树突细胞的激活过程的研究通常需要大量的细胞，这些细胞不可能通过附着于表面而改变或活化，通过在阳性选择期间抗体与表面抗原的结合，或通过释放 的血小板或其他非骨髓细胞在分离或共培养期间的活化剂。 人类外周血单核细胞以及来自相同血液供体的淋巴细胞可以通过逆流淘析使用Lionetti等人（1980）的技术的修改来分离，如前所述（Bobak等人 。，1986）。 从吸入抗凝血剂的单位，可以获得60-120,000个单核细胞。 这些细胞没有被活化并且已经显示在多个研究中适当地能够差别激活。
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