Student Dumont #7 Forceps
Company: Fine Science Tools
Catalog#: 91197-00
Product evaluation:
Craniotomy for Cortical Voltage-sensitive Dye Imaging in Mice
Author:
Date:
2016-02-05
[Abstract]  Cortico-cortical interactions play crucial roles in various brain functions. Here, we present a detailed surgical procedure for cortical voltage-sensitive dye (VSD) imaging that allows monitoring of spatiotemporal dynamics in cortical activity in living mice. Cortical neurons in the upper layers (layer 1-3) are stained with a VSD, and an image sensor with a fast sampling rate (500 Hz) detects fluorescent changes in corrective activity. The procedure includes fixing a mouse brain to a ...

Retinal Explant Culture
Author:
Date:
2014-01-20
[Abstract]  A particularly powerful culture method for the retina is the explant assay, which consists in culturing a small piece of retina on an organotypic filter. Retinal explants can be prepared any time between embryonic day 13 (E13) and postnatal day 4 (P4). Although retinal ganglion cells tend to degenerate shortly after they are generated in explants, and photoreceptor cells do not grow extended outer segments, the explants will develop very similarly to a retina in vivo and generate all ...

Dissociated Retinal Cell Culture
Author:
Date:
2014-01-20
[Abstract]  The retina is a relatively simple and accessible part of the central nervous system, making it a powerful model to study cell fate specification mechanisms. Multipotent retinal progenitor cells (RPCs) give rise to seven major classes of retinal cell types. Mechanisms regulating cell fate choice in the retina depend on both cell intrinsic and environmental factors, but their relative contribution to specific cell fate decisions remains unclear. Dissociated retinal cell cultures provide a great ...

Comments
  • Bio-protocol’s mission is to improve research reproducibility. Please join our effort by sharing your experience on the reagents/equipment that you have used.
Similar products