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Author:
Date:
2013-11-20
[Abstract] Lignin is a complex phenolic polymer deposited in secondarily-thickened plant cell walls. The polymer is mainly derived from the three primary monolignols: p-coumaryl, coniferyl and sinapyl alcohol which give rise to p-hydroxyphenyl, guaiacyl and syringyl units (H, G and S units, respectively) when coupled into the polymer. The building blocks differ in their degree of methoxylation and their biosynthetic pathway is catalyzed by more than 10 enzymes. HCT plays a crucial role by channeling the phenylpropanoids towards the production of coniferyl and sinapyl alcohols. Interestingly, HCT has been reported to be implicated in the pathway both upstream and downstream of the 3-hydroxylation of the aromatic ring of p-coumaroyl shikimate (Figure 1) (Hoffmann et al., ...
[摘要] 木质素是沉积在二次增厚的植物细胞壁中的复杂酚类聚合物。聚合物主要衍生自当偶联时产生对羟基苯基，愈创木基和紫丁香基单元（分别为H，G和S单元）的三种主要单独的木质素：p - 香豆酰基，松柏基和芥子醇进入聚合物。结构单元的甲氧基化程度不同，它们的生物合成途径被超过10种酶催化。 HCT通过将苯丙素类导向松柏醇和芥子醇的生产来发挥关键作用。有趣的是，据报道，HCT涉及p - 香豆酰基莽草酸的芳环的3-羟基化的上游和下游途径（图1）（Hoffmann等， 2003; Hoffmann等人，2004; Vanholme等人，2013b）。这些特征突出了开发在植物中可靠地测量HCT活性的测定法的重要性。在这里，我们描述了基于UPLC-MS的方法，用于分析黑曲霉的木质部总蛋白提取物中的HCT活性，其可以适用于其他木本和草本植物物种。该方案最初在Vanholme等人（2013a）中描述。
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