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Tryptic Soy Broth (TSB)

胰蛋白酶大豆肉汤(TSB)

Company: VWR
Catalog#: EM1.00525.5007
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Shigella IpaD and IpaB Surface Localizations
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2013-11-20
[Abstract]  Shigella uses a type III secretion system to invade host cell and to cause disease. Secretion control and insertion of a translocation pore into cell membrane are critical steps for pathogenesis and are tightly linked to the formation of the needle tip complex formed by the IpaB and IpaD proteins (Veenendaal et al., 2007). Surface localizations of IpaD and IpaB were monitored by FACS analysis according to the localization protocol for Pseudomonas aeruginosa homolog PcrV (Lee et al., 2010). [摘要]  志贺氏菌使用III型分泌系统侵入宿主细胞并引起疾病。 分泌控制和易位孔插入细胞膜是发病的关键步骤,并且与由IpaB和IpaD蛋白形成的针尖复合物的形成紧密相关(Veenendaal等人,2007) 。 根据铜绿假单胞菌同源物PcrV的定位方案通过FACS分析监测IpaD和IpaB的表面定位(Lee等人,2010)。

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