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Dumont #5 - Mirror Finish Forceps, Standard, 0.1 X 0.06Mm

Dumont#5-Mirror Finish Forceps,0.05×0.02mm

Company: Fine Science Tools
Catalog#: 11252-23
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Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
Author:
Date:
2017-10-20
[Abstract]  Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal Sprague-Dawley (SD) rats. In the present protocol, dissociated SCs are isolated from the spinal nerves of neonatal rats and purified by the treatment of cytosine arabinoside (AraC). [摘要]  原代培养的雪旺氏细胞(SCs)广泛用于SC的生物学研究,是神经组织工程的重要种子细胞。 在这里,我们描述了从新生Sprague-Dawley(SD)大鼠中收获原代培养的SC的新方案。 在本方案中,解离的SCs从新生大鼠的脊神经中分离并通过治疗阿糖胞苷(AraC)进行纯化。
【背景】SC是周围神经系统(PNS)的神经胶质细胞。主要SCs的分离和纯化是研究SC生物学的关键步骤。此外,纯化的原代培养的SC是用于神经组织工程的重要种子细胞。迄今为止,已经基于Brockes(Brockes等人,1979)的方法报道了培养SC的各种方法。通过报道的方法,坐骨神经主要用于SC隔离,因为它们尺寸大并且可以容易地获得。然而,坐骨神经的SC容易被成纤维细胞污染,因为结缔组织难以清除。特别是神经管和神经束是成纤维细胞的主要来源。没有特殊处理,污染的成纤维细胞比SCs快得多,并且将很快成为培养物中主要的细胞。在过去几十年中,已经开发出许多用于从污染成纤维细胞中分离SC的纯化方法。这些纯化方法的细节包括单一或组合的抗有丝分裂处理(Wood,1976),抗体介导的细胞溶解(Brockes等人,1979),免疫选择(Assouline等人,,1983; ...

Preparation and Immunofluorescence Staining of the Trachea in Drosophila Larvae and Pupae
Author:
Date:
2016-05-05
[Abstract]  The Drosophila melanogaster trachea is a branched network of rigid chitin-lined tubes that ramify throughout the body and functions as the fly’s respiratory organ. Small openings at the ends of the tracheal tubes allow gas exchange to occur by diffusion between internal tissues and the exterior environment. Tracheal tubes are lined by a single layer of epithelial cells, which secrete chitin and control tube morphology and size. Studies of tracheal development in Drosophila embryos have elucidated fundamental mechanisms of tube morphogenesis and maintenance in vivo, and identified major signaling pathways that regulate these processes (Manning and Krasnow, 1993; Affolter and Shilo, 2000; Zuo et al., 2013; Kerman et al., ... [摘要]   melanogaster 气管是一种由几丁质内衬的管组成的分支网络,分布在整个身体并作为苍蝇的呼吸器官。气管管端部的小开口允许通过内部组织和外部环境之间的扩散而发生气体交换。气管管由单层上皮细胞衬里,其分泌几丁质和对照管形态和尺寸。在Drosop hila 胚胎中的气管发育的研究阐明了管形态发生和维持体内的基本机制,并且鉴定了调节这些过程的主要信号传导途径(Manning和Krasnow,1993; Affolter和Shilo,2000; Zuo等人,2013; Kerman等人,2006; Schottenfeld等人, em,2010)。近年来,在变态过程中对气管的兴趣日益增长,当在幼虫中用作呼吸器官的气管分支被修复或被由致动的气管祖细胞产生的新的气管组织或成熟的气管细胞(Manning和Krasnow,1993; Sato和Kornberg,2002; Guha等人,2008; Guha和Kornberg,2005; Weaver和Krasnow,2008; Pitsouli和Perrimon, 2010; Chen和Krasnow,2014)在过程结束时形成成人气管。正在进行的衰变和组织形成模拟其他生物体中组织修复和再生的方面,并且已经用于理解祖细胞如何分裂和分化(Pitsouli和Perrimon,2010; ...

Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
Author:
Date:
2016-04-05
[Abstract]  We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015). [摘要]  我们在这里提供了详细的协议,用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节(DRG)中。 解离的小鼠DRG可以通过酶消化解离成单个细胞,以获得小鼠感觉神经元的原代培养物,如Khaminets等人(2015)报道的研究中所进行的。

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