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Author:
Date:
2013-04-20
[Abstract] In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bindler et al., 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.
[摘要] 在该方案中，描述了通过微卫星标记在烟草中确定种子亲子。 然而，这不包括新标记选择/鉴定的方案，而是利用为密切相关物种N产生的标记。 tabacum （Bindler ，2007）。 这是一种高通量方案，对于技术人员来说，优化和流线化以在5天内从微生物分离（从幼苗）到通过微卫星基因型数据的亲子鉴定来处理384（96×4）种子。
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