| Retinal Differentiation of Mouse Embryonic Stem Cells
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Author:
Date:
2016-07-05
[Abstract] Groundbreaking studies from Dr. Yoshiki Sasai’s laboratory have recently introduced novel methods to differentiate mouse and human Embryonic Stem Cells (mESCs and hESCs) into organ-like 3D structures aimed to recapitulate developmental organogenesis programs (Eiraku et al., 2011; Eiraku and Sasai, 2012; Nakano et al., 2012; Kamiya et al., 2011). We took advantage of this method to optimize a 3D protocol to efficiently generate retinal progenitor cells and subsequently retinal neurons in vitro. This culture system provides an invaluable platform both to study early developmental processes and to obtain retinal neurons for transplantation approaches. The protocol described here has been successfully applied to several mouse ESC (including the R1, WD44 and ...
[摘要] 来自Yoshiki Sasai博士的实验室的开创性研究最近已经引入了将小鼠和人胚胎干细胞（mESC和hESC）区分为器官样3D结构的新方法，其旨在重现发育器官发生程序（Eiraku等人， ，2011; Eiraku和Sasai，2012; Nakano等人，2012; Kamiya等人，2011）。 我们利用这种方法优化3D协议以有效地生成视网膜祖细胞和随后视网膜神经元体外。 这种文化系统提供了一个宝贵的平台，既研究早期发展过程，并获得视网膜神经元的移植方法。 这里描述的协议已成功应用于几个鼠标ESC（包括R1，WD44和G4细胞系）和小鼠诱导多能干细胞（iPSCs）线。
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| Glioma Associated Stem Cells (GASCs) Isolation and Culture
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Author:
Date:
2015-05-05
[Abstract] Glioma Associated Stem Cells (GASCs) represent a population of non-tumorigenic multipotent stem cells hosted in the microenvironment of human gliomas. In vitro, these cells are able, through the release of exosomes, to increase the biological aggressiveness of glioma-initiating cells. The clinical importance of this finding is supported by the strong prognostic value associated with the GASCs surface immunophenotype thus suggesting that this patient-based approach can provide a groundbreaking method to predict prognosis and to exploit novel strategies that target the tumor stroma (Bourkoula et al., 2014).
[摘要] 胶质瘤相关干细胞（GASC）代表在人类神经胶质瘤的微环境中存在的非致瘤性多能干细胞群体。 在体外，这些细胞能够通过外来体的释放增加胶质瘤起始细胞的生物攻击性。 该发现的临床重要性得到与GASCs表面免疫表型相关的强预后价值的支持，因此表明这种基于患者的方法可以提供开创性的方法来预测预后并开发靶向肿瘤基质的新策略（Bourkoula等人， et al。，2014）。
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| Culturing of C57BL/6 Mouse Embryonic Stem (ES) Cell Line
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Author:
Date:
2011-10-05
[Abstract] Using ATCC ES C57BL/6 as an example, it is shown here how to culture mouse embryonic stem (ES) cell line. The clonal embryonic stem cell line #693 ES C57BL/6 was derived from a strain C57BL/6J (B6) mouse blastocyst [PubMed: 11730008]. The ES cells were shown to populate the germ line of two host blastocyst donors, FVB/NJ (FVB) and the coisogenic strain C57BL/6-Tyrc-2J (c2J). Coat-color chimera production was high using c2J blastocysts while FVB blastocysts produced a low number of chimeras [PubMed: 11730008].
[摘要] 使用ATCC ES C57BL/6作为实例，在此显示如何培养小鼠胚胎干（ES）细胞系。 克隆胚胎干细胞系＃693ES C57BL/6源自菌株C57BL/6J（B6）小鼠胚泡[PubMed：11730008]。 ES细胞显示为填充两个宿主胚泡供体FVB/NJ（FVB）和同种型菌株C57BL/6-Tyrc-2J（c2J）的种系。 使用c2J胚泡的外套嵌合体产量高，而FVB胚泡产生低数量的嵌合体[PubMed：11730008]。
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