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Cell culture CO2 incubator

细胞培养CO2培养箱

Company: Panasonic Healthcare
Catalog#: MCO-20AIC
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Efficient Isolation of Influenza Specific CTLs
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Date:
2016-06-20
[Abstract]  Human antigen-specific CD8+ T-cell clones are valuable tools for dissecting CD8+ T-cell responses against antigens derived from infectious agents, cancer and self antigens. Here we describe a protocol for isolating human antigen-specific CD8+ T cells. This protocol uses surface capture of IFNγ to identify antigen responsive cells that are then cloned by single-cell sorting. Here we use CD8+ T-cell responses to influenza matrix protein (MP) as an example, but this approach can be applied to any antigen specificity. [摘要]  人抗原特异性CD8 + T细胞克隆是用于解剖来自感染剂,癌症和自身抗原的抗原的CD8 + T细胞应答的有价值的工具。 在这里我们描述了用于分离人抗原特异性CD8 + T细胞的方案。 该协议使用IFNγ的表面捕获来鉴定抗原反应性细胞,然后通过单细胞分选克隆。 这里我们使用CD8 + T细胞对流感基质蛋白(MP)的反应作为例子,但这种方法可以应用于任何抗原特异性。

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