GibcoTM Amphotericin B
Company: Thermo Fisher Scientific
Catalog#: 15290026
Product evaluation:
Heterochronic Pellet Assay to Test Cell-cell Communication in the Mouse Retina
Author:
Date:
2017-02-05
[Abstract]  All seven retinal cell types that make up the mature retina are generated from a common, multipotent pool of retinal progenitor cells (RPCs) (Wallace, 2011). One way that RPCs know when sufficient numbers of particular cell-types have been generated is through negative feedback signals, which are emitted by differentiated cells and must reach threshold levels to block additional differentiation of that cell type. A key assay to assess whether negative feedback signals are emitted by ...

Ex vivo Culture of Adult Mouse Antral Glands
Author:
Date:
2017-01-05
[Abstract]  The tri-dimensional culture, initially described by Sato et al. (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker et al. (2010), who efficiently generated organoids that recapitulate the mature pyloric epithelium in vitro. This ex vivo approach is suitable and promising to ...

Ex vivo Culture of Fetal Mouse Gastric Epithelial Progenitors
Author:
Date:
2017-01-05
[Abstract]  Isolation and tridimensional culture of murine fetal progenitors from the digestive tract represents a new approach to study the nature and the biological characteristics of these epithelial cells that are present before the onset of the cytodifferentiation process during development. In 2013, Mustata et al. described the isolation of intestinal fetal progenitors growing as spheroids in the ex vivo culture system initially implemented by Sato et al. (2009) to grow ...

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