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Author:
Date:
2016-03-20
[Abstract] This assay allows to determine whether proteins possess oxidative activity-the ability to introduce disulfide bond in vitro. The substrate for potential oxidases is a ribonuclease A which, for its activity, needs 4 properly formed disulfide bonds (Raines, 1998).
RNase A activity can be detected by:
- Monitoring the digestion of RNA (Lambert and Freedman, 1983);
- Methylene Blue assay (Greiner-Stoeffele et al., 1996);
- Analyzing the cleavage of the cyclic CMP (Lyles and Gilbert, 1991; Lyles and Gilbert, 1991).
We here describe method for measurements of oxidative activity, based on the cleavage of cCMP.
Oxidative activity will be tested by measuring spectrophotometrically RNase A cleavage of cyclic-2’, ...
[摘要] 该测定允许确定蛋白质是否具有氧化活性 - 在体外引入二硫键的能力。 潜在氧化酶的底物是核糖核酸酶A，其活性需要4个适当形成的二硫键（Raines，1998）。
核糖核酸酶A活性可通过以下检测：
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