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Author:
Date:
2016-03-05
[Abstract] Pollen grains are male gametophytes produced within the pollen sacs of the anthers of the flower. Recent genetic studies have revealed several components involved in microspore development (Borg et al., 2009; Berger and Twell, 2011), and yet many components controlling microspore development remain to be identified. Semi-thin sectioning of anthers and light and fluorescence microscopy of floral bud (Kim et al., 2015) are the initial key experiments to characterize Arabidopsis mutants and transgenic plants for understanding the roles of new genetic components during microspore development. Herein, we describe a protocol for semi-thin sectioning of anthers and light and fluorescence microscopy of floral bud in Arabidopsis.
[摘要] 花粉粒是在花的花药的花粉囊内产生的雄性配子体。 最近的遗传研究揭示了涉及小孢子发育的几种组分（Borg等人，2009; Berger和Twell，2011），然而仍有许多控制小孢子发育的组分被鉴定。 花药的半薄切片和花芽的光和荧光显微镜检查（Kim等人，2015）是表征拟南芥突变体和转基因植物的初始关键实验 在小孢子发育过程中新遗传组分的作用。 在这里，我们描述了拟南芥花药半花瓣和光和荧光显微镜的半薄切片的协议。
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