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Author:
Date:
2016-03-05
[Abstract] Dendritic cells (DCs) play a critical role in mounting the T cell response against different infectious agents. Nature and intensity of the induced T cell responses are defined by activation status of DCs. It is generally accepted that IL-12, IL-4/IL-5 and IL-23 producing DCs induce TH1, TH2 and TH17 type of immune responses, respectively (Kumar et al., 2015). Besides cytokines, levels of co-stimulatory molecules on DCs also influence the response of T cells.
The activation status of DCs can be determined by examining DC culture supernatants for different cytokines and by analyzing expression of co-stimulatory molecules on these cells. However, these approaches provide indirect information about T cell activating potential of DCs. ...
[摘要] 树突细胞（DC）在安装针对不同感染剂的T细胞应答中起关键作用。诱导的T细胞应答的性质和强度由DC的活化状态定义。通常接受的是，产生IL-12，IL-4/IL-5和IL-23的DC诱导T H 1，T H 2和T H （Kumar等人，2015年）中的免疫应答类型。除了细胞因子，DC上的共刺激分子的水平也影响T细胞的响应。通过检查不同细胞因子的DC培养上清液并通过分析这些细胞因子上的共刺激分子的表达，可以确定DC的活化状态细胞。然而，这些方法提供关于DC的T细胞活化潜能的间接信息。在共培养系统中分析T细胞应答是更直接的方法来检查DC的T细胞增殖和极化功效。
在同种异体混合白细胞反应中分析DC的T细胞增殖和极化潜能的方案（allo-MLR）。
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