{{'Search' | translate}}
 

DPBS

DPBS

Company: Thermo Fisher Scientific
Catalog#: 14190235
Bio-protocol()
Company-protocol()
Other protocol()

Whole-mount Enteroid Proliferation Staining
Author:
Date:
2016-06-20
[Abstract]  Small intestinal organoids, otherwise known as enteroids, have become an increasingly utilized model for intestinal biology in vitro as they recapitulate the various epithelial cells within the intestinal crypt (Mahe et al., 2013; Sato et al., 2009). Assessment of growth dynamics within these cultures is an important step to understanding how alterations in gene expression, treatment with protective and toxic agents, and genetic mutations alter properties essential for crypt growth and survival as well as the stem cell properties of the individual cells within the crypt. This protocol describes a method of visualization of proliferating cells within the crypt in three dimensions (Barrett et al., 2015). Whole-mount proliferation staining of enteroids ... [摘要]  小肠类器官,也称为肠袢,已经成为越来越多地用于肠道生物学的体外模型,因为它们重现了在肠隐窝内的各种上皮细胞(Mahe等人 ,2013; Sato et al。,2009)。在这些培养物中评估生长动力学是理解基因表达的改变,用保护性和毒性试剂处理以及遗传突变改变隐窝生长和存活所必需的性质以及细胞内单个细胞的干细胞性质的重要步骤地穴。该方案描述了在隐窝内三维的增殖细胞的可视化方法(Barrett等人,2015)。使用EdU掺入的肠衣的整体增殖染色使得研究者能够观察到肠内的所有增殖细胞,而不是如在包埋和切片中所见到的在薄切片中获得生长信息,确保来自干细胞区室的增殖的真实表现到隐窝的终末分化细胞。

Preparation of Synovial Mesenchymal Stem Cells from a Rat Knee Joint
Author:
Date:
2016-05-05
[Abstract]  Mesenchymal stem cells (MSCs), first described in human bone marrow, are emerging as promising cell-based therapeutics for a wide range of diseases (Caplan and Correa, 2011). MSCs have been isolated from various organs in the body, and synovial MSCs were first reported by De Bari et al. (2001). We previously reported that synovial MSCs have superior proliferation and chondrogenic potentials as compared to bone marrow-, muscle-, and adipose- derived MSCs in humans (Sakaguchi et al., 2005) and rats (Yoshimura et al., 2007). In addition, administration of synovial MSCs for osteochondral defect promoted cartilage regeneration in a rabbit (Koga et al., 2008) and a pig model (Nakamura et al., 2012). In 2008, we started a clinical trial in human and ... [摘要]  首先在人骨髓中描述的间充质干细胞(MSC)正在作为用于广泛疾病的有希望的基于细胞的治疗剂出现(Caplan和Correa,2011)。 MSC已经从体内各种器官中分离,并且滑膜MSCs首先由De Bari等人(2001)报道。我们先前报道了与人类骨髓,肌肉和脂肪来源的MSC(Sakaguchi等人,2005)和大鼠(Yoshimura等人,2005)相比,滑膜MSC具有更好的增殖和软骨形成潜能> et al。,2007)。此外,施用用于骨软骨缺损的滑膜MSC促进兔子中的软骨再生(Koga等人,2008)和猪模型(Nakamura等人,2012) 。在2008年,我们开始了人类的临床试验,并通过磁共振成像获得令人满意的症状和再生软骨的结果(Sekiya等人,2015)。我们还利用滑膜MSC在大鼠中进行了半月板再生的多个研究线(Horie等人,2009; Horie等人,2012; Katagiri等人al。,2013; Okuno等人,2014; Ozeki等人,2015)。在本文中,我们演示了如何从大鼠膝关节收获包括髌下脂肪垫的滑膜,并描述大鼠滑膜MSC的分离和培养技术。

Comments