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Author:
Date:
2015-12-20
[Abstract] Leptolyngbya boryana (L. boryana) (formerly Plectonema boryanum) is a versatile, filamentous cyanobacterium that has the ability to fix nitrogen under microoxic conditions and to grow heterotrophically with glucose in the dark, providing an excellent system to investigate photosynthesis, nitrogen fixation, and their regulatory mechanisms. While L. boryana is not naturally transformable different from the unicellular cyanobacterium Synechocystis sp. PCC 6803, it can be transformed by electroporation. Here we describe the transformation of L. boryana by electroporation to isolate mutants in which a targeted gene is disrupted.
[摘要] Leptolyngbya boryana （ L。boryana ）（以前称为Plectonema boryanum）是一种多用途的丝状蓝细菌，具有在微氧条件下固氮的能力， 在黑暗中与葡萄糖异养生长，提供了研究光合作用，固氮和它们的调节机制的优良系统。 而 L。 boryana 不是天然可转化的，不同于单细胞蓝细菌集胞藻。 PCC 6803，它可以通过电穿孔转化。 这里我们描述了 L的变换。 boryana通过电穿孔分离其中靶基因被破坏的突变体。
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