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Author:
Date:
2015-10-20
[Abstract] Hyaloperonospora arabidopsidis (Hpa; formerly Peronospora parasitica or Hyaloperonospora parasitica) is an oomycete downy mildew pathogen of the model plant Arabidopsis. The pathosystem between Arabidopsis and Hpa has been extensively used to study host/pathogen co-evolution (Coates and Beynon, 2010). As Hpa is an obligate biotrophic pathogen, its host is absolutely required for survival. Thus, Hpa must be maintained on susceptible Arabidopsis accessions and mutants. Growth of Hpa is evaluated in two ways; counting conidiospores (Asai et al., 2014) or counting sporangiophores after trypan blue staining (Holt et al., 2005). Here, we describe how to do inoculation with Hpa ...
[摘要] （ Hpa ;以前为寄生寄生霜霉菌或寄生寄生虫）是模式植物的卵菌霜霉病病原体 拟南芥。 拟南芥和 Hpa之间的细胞系统已广泛用于研究宿主/病原体共进化（Coates和Beynon，2010）。 作为Hpa 是一种专性的生物营养性病原体，其宿主是生存所必需的。 因此，必须在易感的拟南芥登录号和突变体上维持 Hpa 。 生长Hpa 以两种方式评估： （Asai等人，2014）或在台盼蓝染色后计数孢子生成（Holt等人，2005）。 在这里，我们描述了如何用 Hpa 进行接种以及如何在拟南芥上评估 Hpa
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