|
Author:
Date:
2015-10-20
[Abstract] Collagen-induced arthritis (CIA) is a common autoimmune animal model used to study rheumatoid arthritis (RA). The development of CIA involves infiltration of macrophages and neutrophils into the joint, as well as T and B cell responses to type II collagen. In murine CIA, genetically susceptible mice (DBA/1J) are immunized with a type II bovine collagen emulsion in complete Freund’s adjuvant (CFA), and receive a boost of type II bovine collagen in incomplete Freund’s adjuvant (IFA) 21 days after the first injection. These mice typically develop disease 26 to 35 days after the initial injection. C57BL/6J mice are resistant to arthritis induced by type II bovine collagen, but can develop arthritis when immunized with type II chicken collagen in CFA, and receive a boost of type II chicken ...
[摘要] 胶原诱导的关节炎（CIA）是用于研究类风湿性关节炎（RA）的常见的自身免疫动物模型。 CIA的发展涉及巨噬细胞和嗜中性粒细胞渗入关节，以及T和B细胞对II型胶原的反应。在鼠CIA中，用完全弗氏佐剂（CFA）中的II型牛胶原乳剂免疫遗传易感小鼠（DBA/1J），并在第一次给药21天后接受不完全弗氏佐剂（IFA）中II型牛胶原的加强免疫注射。这些小鼠通常在初次注射后26至35天发展疾病。 C57BL/6J小鼠对由II型牛胶原蛋白诱导的关节炎具有抗性，但当在CFA中用II型鸡胶原免疫时可产生关节炎，并且在首次注射后21天在IFA中接受II型鸡胶原蛋白的加强。热死亡的结核分枝杆菌H37RA（MT）在CFA中的浓度对于每个菌株也不同。 DBA/1J小鼠发展具有1mg/ml MT的关节炎，而C57BL/6J小鼠需要和3-4mg/ml MT以形成关节炎。 CIA在C57BL/6J小鼠中缓慢发展，并且与DBA/1J小鼠相比，关节炎病例轻度。该方案描述了用II型牛胶原免疫DBA/1J小鼠和用II型鸡胶原免疫C57BL/6J小鼠。
|