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Sodium chloride solution

氯化钠溶液

Company: Sigma-Aldrich
Catalog#: S8776
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Mouse Satellite Cell Isolation and Transplantation
Author:
Date:
2018-01-20
[Abstract]  Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs (Reano et al., 2017). [摘要]  卫星细胞(SC)移植代表了肌肉再生期间SC生物学研究的强大策略。 我们在这里描述了从绿色荧光蛋白(GFP)表达的小鼠和他们的小鼠肌肉移植SC分离的协议。 该程序最初用于评估激素非酰化生长素释放肽对肌肉再生的影响,特别是评估受体肌肉中未酰化的生长素释放肽的增加如何影响供体SC的植入(Reano等人,2017年)。

【背景】由分化的肌纤维组成的骨骼肌可以在受伤时再生。肌肉再生依赖于居住在肌肉纤维基底层之下的称为卫星细胞(SC)的静止驻留干细胞群(Mauro,1961)。受伤后,SC经历激活,广泛的增殖,分化和融合,最终修复或替换受损的肌纤维(Collins等人,2005)。

由于移植的成肌细胞可以与宿主成肌细胞融合,因此SC的移植被认为是杜氏肌营养不良症(DMD)的潜在疗法多年的时间,这提示了在缺陷纤维中功能性修复的可能性(Partridge ...

Study of Epithelium Barrier Functions by Real-time TER Measurement
Author:
Date:
2016-05-20
[Abstract]  Transepithelial Electrical Resistance (TER) measurement is a reliable and efficient method to quantify the permeability of barrier forming cells such as epithelial cells. Measuring the permeability of the epithelial cells will help the researchers to investigate the barrier function of epithelium in various infectious and inflammatory diseases. Here we provide a real-time and impedance-based approach for measuring the permeability of epithelial cell monolayer using the Electrical Cell Substrate Impedance Sensing (ECIS®) instrumentation. [摘要]  经上皮电阻(TER)测量是量化屏障形成细胞例如上皮细胞的渗透性的可靠和有效的方法。 测量上皮细胞的通透性将有助于研究人员调查上皮在各种感染和炎症疾病中的屏障功能。 在这里,我们提供了使用电细胞底物阻抗感测(ECIS )仪器测量上皮细胞单层的渗透性的基于实时和阻抗的方法。

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