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Company: Eppendorf

Catalog#: 30089618

Profiling of Single-cell-type-specific MicroRNAs in Arabidopsis Roots by Immunoprecipitation of Root Cell-layer-specific GFP-AGO1

Lusheng Fan, Bin Gao and Xuemei Chen

Published online: 2022-12-20

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Mitochondrial RNA Transcript Analysis Assay of Arabidopsis Leaf Tissues

Author:

Etienne Delannoy, Andéol Falcon de Longevialle and Catherine Colas des Francs-Small,

Date:

2015-10-20

[Abstract] This qPCR-based assay provides an overview of the expression levels of all mitochondrial transcripts (mRNAs and rRNAs) as well as splicing efficiency in Arabidopsis. It was developed before RNAseq techniques were widely used (de Longevialle et al., 2007), but is nevertheless still useful as it is cheaper to run and the analysis is much easier and faster to perform if the aim is only to look at mitochondrial transcripts. For intron-containing mRNAs, the use of primer sets specifically amplifying spliced or unspliced forms allows the evaluation of the splicing efficiency. [摘要] 这种基于qPCR的测定提供了所有线粒体转录物（mRNA和rRNA）的表达水平以及拟南芥中的剪接效率的概述。它是在RNAseq技术被广泛使用之前开发的（de Longevialle等人，2007），但是仍然有用，因为它运行更便宜，并且分析更容易和更快地执行，如果目标只是看看线粒体转录物。对于含内含子的mRNA，使用特异性扩增剪接或未剪接形式的引物组允许评价剪接效率。

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Combitips 0.1 ml

Combitips 0.1 ml

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