| Soluble and Solid Iron Reduction Assays with Desulfitobacterium hafniense
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Author:
Date:
2018-09-05
[Abstract] There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict anaerobe Desulfitobacterium hafniense (strains TCE1 and LBE). We investigated iron reduction using three different Fe(III) sources: Fe(III)-citrate (a soluble phase), akaganeite (solid iron phase), and corroded coupons. This protocol describes a method that combines spectrophotometric quantification of the complex Fe(II)-Ferrozine® with mineral characterization by scanning electron microscopy and Raman spectroscopy. These three ...
[摘要] 迫切需要开发可持续和有效的方法来保护和稳定铁制物体。为了开发腐蚀铁物体的保护 - 恢复方法，该生物方案提出了研究严格厌氧菌[Desulfitobacterium hafniense （菌株TCE1）中三价铁的还原溶解和稳定亚铁矿物质的生物产生的步骤。和LBE）。我们使用三种不同的Fe（III）来源研究了铁还原：Fe（III） - 柠檬酸盐（可溶相），akaganeite（固体铁相）和腐蚀的试样。该协议描述了一种方法，该方法结合了复杂的Fe（II）-Ferrozine ®的分光光度定量，通过扫描电子显微镜和拉曼光谱进行矿物表征。这三种方法可以评估三价铁的还原溶解和生物矿物质生产，作为开发一种创新的可持续方法来稳定腐蚀铁的有希望的替代方法。
【背景】自铁器时代以来，铁已被用于生产日常用具。因此，考古学上的铁试验是过去极其重要的证据，应予以保留。然而，由于其反应性，铁容易被腐蚀并且考古铁物体可能被完全损坏。埋藏时，铁制品会根据埋葬地点的环境条件形成复杂的腐蚀层。挖掘后，条件发生变化，腐蚀层变得不稳定。为避免完全破坏，考古铁制物需要快速稳定处理。目前，可用的稳定化处理不能提供长期保护并且具有实质性缺点，例如毒性，低效率和大量废物的产生（Scott和Eggert，2009; Rimmer 等人， 2012）。因此，有必要开发新技术来稳定考古铁器。
越来越多地考虑利用微生物代谢来开发更有效，可持续和环保的保护 ...
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| In vitro Studies: Inhibition of Nevirapine Metabolism by Nortriptyline in Hepatic Microsomes
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Author:
Date:
2015-10-05
[Abstract] One of the most prevalent and interfering psychosocial comorbidities of HIV infection is clinical depression (22 to 45%). For this reason, a study of a possible interaction between the nonnucleoside reverse transcriptase inhibitor nevirapine (NVP) and the tricyclic antidepressant nortriptyline (NT) was carried out. In vitro studies with rat and human hepatic microsomes showed a marked inhibition of NVP metabolism by NT being more intense in rat than in human. The extrapolation of these results to humans suggests increased NVP side effects when both drugs are coadministered, but additional in vivo human studies are required to evaluate the clinical implication of this interaction.
This protocol describes a technique for detecting and measuring the inhibition of ...
[摘要] HIV感染的最普遍和干扰的心理社会共病是临床抑郁症（22％至45％）。 为此，进行了非核苷逆转录酶抑制剂奈韦拉平（NVP）和三环抗抑郁药去甲替林（NT）之间可能的相互作用的研究。 使用大鼠和人肝微粒体的体外研究显示在大鼠中NT比在人中更强烈的NVP代谢的显着抑制。 将这些结果外推到人表明当两种药物共同给药时，NVP副作用增加，但是需要另外的体内人体研究来评价这种相互作用的临床意义。
该方案描述了 检测和测量肝微粒体中去甲替林对奈韦拉平代谢的抑制的技术。
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