Alizarin Red S
Company: Sigma-Aldrich
Catalog#: A5533
Product evaluation:
Binding to Secreted Bone Matrix in vitro
[Abstract]  This method examines the bone matrix binding capacity of proteins. Using osteogenic differentiation medium, multipotent stromal cells (MSC) are induced to differentiate into osteocytes in vitro and to secrete bone matrix. The latter is confirmed using Alizarin red S staining, which detects the presence of calcific deposits (hydroxyapatite). These calcific deposits are used to test the bone binding properties of proteins. The binding to the calcific deposits is assessed by Western blot ...

In vitro Biomineralization Assay
[Abstract]  Biomineralization in vertebrates has both physiological and pathological aspects. Physiological mineralization is essential for proper development and function of hard tissues, such as bone, teeth, and growth plate cartilage, but it does not occur in soft tissues. Pathological ectopic mineralization, in contrast, occurs in soft tissues, including blood vessels, kidney, articular cartilage, and cardiovascular tissue. Here, we describe the simple method for detecting and measuring the presence of ...

Osteogenic and Adipogenic Differentiation of Osteosarcoma Cells
[Abstract]  Osteosarcomas are the most common primary, non-hematologic malignant tumors in childhood and adolescence, comprising almost 60% of all bone sarcomas. Although these tumors are osteoblastic in nature, the characteristics of the specific tumor-initiating cells are unclear. Osteosarcomas contain highly proliferative undifferentiated malignant cells with a disrupted bone differentiation program. Cancer stem cells (CSCs) that have tumor-initiating properties and capacity of symmetric and asymmetric ...

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