| Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
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Author:
Date:
2016-09-05
[Abstract] Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for salinity and drought treatments, the soil-culture method in this study was more applicable to studying natural stress conditions.
[摘要] 盐度和干旱是导致红麻产量和质量损失的两个主要因素。 据报道，盐度和干旱可影响10％以上的耕地，导致主要作物平均单产下降50％以上。 因此，了解植物对干旱和盐分的耐受性具有根本重要性，已成为深入研究的重点。 该协议描述了在土壤条件下模拟天然盐度和干旱胁迫的简单和可重复的方案。 即使水培法最常用于盐分和干旱处理，本研究中的土壤培养方法更适用于研究自然胁迫条件。
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| Pot Level Drought Stress Tolerance Assay in Tobacco through Plant Phenotyping and Antioxidant Assay
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Author:
Date:
2015-10-05
[Abstract] Drought is an important abiotic factor which has a huge detrimental impact on crop productivity. Study of plant responses towards drought stress and investigating the mechanism of drought tolerance is crucial for achieving the target of developing drought-tolerant plants. Phenotyping is a cost effective approach which can be adopted to evaluate the severity of drought stress in a plant. Next to phenotyping parameters, biochemical parameters such as the study of antioxidant enzyme activity play significant roles in assessing the extent of drought stress caused injury in a plant. Among the antioxidant enzymes, ascorbate peroxidase is an enzyme which plays a crucial role in drought tolerance in plants. It has been well established that the activity of this enzyme increases under drought ...
[摘要] 干旱是一个重要的非生物因素，对作物生产力有巨大的不利影响。研究植物对干旱胁迫的反应和研究干旱耐受机制对于实现发展耐旱植物的目标是至关重要的。表型是一种成本有效的方法，可用于评价植物中干旱胁迫的严重性。除了表型参数之外，生物化学参数例如抗氧化酶活性的研究在评估植物中干旱胁迫引起的损伤的程度中起重要作用。在抗氧化酶中，抗坏血酸过氧化物酶是在植物的耐旱性中起关键作用的酶。已经确定该酶的活性在干旱胁迫下增加。在这里，我们提出了一个简单和可重复的协议，以调查烟草植物对干旱胁迫通过测量表型参数和抗氧化酶活性的反应。虽然，这些实验已经进行了烟草植物，这个协议可以采用其他作物品种。
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