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TES

TES

Company: Carl Roth
Catalog#: 9137.3
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Spot Assays for Viability Analysis of Cyanobacteria
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2015-09-05
[Abstract]   Cyanobacteria are prokaryotic organisms performing oxygenic photosynthesis. The cyanobacterium Synechocystis sp. PCC 6803 is a model organism for the study of photosynthesis, gene regulation and biotechnological applications because it is easy to manipulate genetically. Moreover, this cyanobacterium can grow photoautotrophically as well as chemoheterotrophically in the dark utilizing glucose. Microbiologists often use optical density measured with a spectrophotometer for the comparison of growth performance of different strains in liquid cultures. Because Synechocystis sp. PCC 6803 (especially motile strains) tend to form aggregates under stress conditions this method might be not suitable for evaluation of different strains under different growth conditions. ... [摘要]  蓝细菌是进行含氧光合作用的原核生物。蓝细菌集胞藻 PCC 6803是一种用于光合作用,基因调控和生物技术应用研究的模式生物,因为它易于基因操作。此外,这种蓝细菌可以在黑暗中利用葡萄糖光化自养地以及化学异养生长。微生物学家经常使用用分光光度计测量的光密度用于比较液体培养物中不同菌株的生长性能。因为 Synechocystis PCC 6803(特别是运动株)倾向于在胁迫条件下形成聚集体,该方法可能不适合在不同生长条件下评估不同的菌株。此外,许多实验室不能很好地配备标准化的光生物反应器和照明设施,以确保生长曲线的可重复性。在这里,我们描述了高度可重复的斑点测定的蓝细菌菌株的生存力分析。

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