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GLYCEROL 99,5%

GLYCEROL 99,5%

Company: GERBU Biotechnik
Catalog#: 2006
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Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
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2015-08-05
[Abstract]  The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E. coli) and subsequent affinity purification using a maltose binding protein (MBP) tag. The proteins can be used in SUMOylation reactions, where the MBP part of the protein can be detected with a commercially available antibody, or additional purification steps can be applied. [摘要]  蛋白质PIAL1(At1g08910)和PIAL2(At5g41580)是最近发现的植物E4 SUMO连接酶的成员。 该方案允许在大肠杆菌(大肠杆菌)中快速和简单地表达重组蛋白,并随后使用麦芽糖结合蛋白(MBP)标签进行亲和纯化。 蛋白质可用于SUMO化反应中,其中蛋白质的MBP部分可用市售的抗体检测,或可应用额外的纯化步骤。

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