34-1-2S

Company: ATCC

Catalog#: HB-79^TM

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Two-event Transfusion-related Acute Lung Injury Mouse Model

Author:

Guadalupe Ortiz-Muñoz and Mark R. Looney,

Date:

2015-06-20

[Abstract] Transfusion-related acute lung injury (TRALI) is defined as acute lung injury that occurs within 6 hours of a blood product transfusion. TRALI continues to be a leading cause of transfusion-related mortality and we have developed a mouse model of TRALI to better understand the mechanisms by which injury occurs and to test therapeutic approaches. Our model is a two-event model based on immune priming and the challenge of BALB/c wild-type mice with cognate MHC Class I monoclonal antibody (MHC I mAb). Immune priming with LPS mimics the primed state of recipients (first event) that is important for the development of TRALI. Donor HLA antibodies are frequently implicated in TRALI reactions, and cognate MHC Class I antibody (second event) produces acute lung injury in primed animals. Here, we ... [摘要] 输血相关的急性肺损伤（TRALI）定义为在血液制品输血的6小时内发生的急性肺损伤。 TRALI仍然是输血相关死亡率的主要原因，我们已经开发了TRALI的小鼠模型，以更好地了解损伤发生的机制和测试治疗方法。我们的模型是基于免疫引发的双事件模型和具有同源MHC I类单克隆抗体（MHC I mAb）的BALB/c野生型小鼠的攻击。用LPS的免疫引发模拟对TRALI的发展重要的受体的引发状态（第一事件）。供体HLA抗体经常涉及TRALI反应，并且同源MHC I类抗体（第二事件）在引发的动物中产生急性肺损伤。在这里，我们描述一个详细的协议，在动物中具有高重现性。

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34-1-2S

34-1-2S

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