| Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
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Author:
Date:
2015-06-05
[Abstract] Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including EBV) and this HIV reservoir (Gianella et al., 2014). This protocol was designed to simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell (PBMC) aliquot and quantify HIV, CMV and EBV DNA, as well as HIV RNA using droplet digital PCR (ddPCR).
For collection and processing of male genital secretions and quantification of HIV RNA and DNA from seven human herpesviruses from seminal plasma, refer to ...
[摘要] 几乎所有感染人免疫缺陷病毒（HIV）的个体也被巨细胞病毒（CMV）和EB病毒（EBV）感染。 我们的研究的目的包括表征和测量潜伏的HIV库并且理解CMV（和其他疱疹病毒，包括EBV）的无症状复制与该HIV库之间的关联（Gianella等人，2014） 。 该方案设计为从相同的外周血单核细胞（PBMC）等分试样同时共提取DNA和RNA，并使用液滴数字PCR（ddPCR）定量HIV，CMV和EBV DNA以及HIV RNA。
关于男性生殖器分泌物的收集和处理以及来自七种人疱疹病毒的HIV RNA和DNA的定量，参见方案"Vacs RNA和人疱疹病毒DNA在Seminal血浆中的定量（Vargas-Meneses等人，2015）。
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| Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma
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Author:
Date:
2015-05-05
[Abstract] Multiple viruses can co-infect the genital tract, modifying the immunologic and virologic milieu and possibly playing a role in viral transmission and pathogenesis. The aim of our studies has been to understand the complex relationships between HIV-1 RNA, and multiple human herpesviruses known to frequently replicate in the genital tract of HIV-infected men (i.e. cytomegalovirus [CMV], Epstein Bar virus [EBV], herpes simplex virus [HSV] types 1 and 2, and human herpesviruses [HHV] 6, 7 and 8) (Gianella et al., 2013a; Gianella et al., 2013b; Gianella et al., 2013c; Gianella et al., 2014). This protocol was designed to collect and process male genital secretion (GS), and to isolate and further quantify HIV RNA and DNA of seven HHV from seminal ...
[摘要] 多种病毒可以共感染生殖道，修改免疫学和病毒学环境，并可能在病毒传播和发病中起作用。 我们的研究的目的是了解HIV-1 RNA和已知在HIV感染的男性生殖道中经常复制的多种人疱疹病毒之间的复杂关系（即巨细胞病毒[CMV]，Epstein 条形病毒[EBV]，单纯疱疹病毒[HSV] 1型和2型，以及人疱疹病毒[HHV] 6,7和8）（Gianella等人，2013a; Gianella等人 。，2013b; Gianella 等人，2013c; Gianella ，2014）。 该方案设计用于收集和处理男性生殖器分泌（GS），并使用定量实时PCR技术从精浆中分离并进一步定量7个HHV的HIV RNA和DNA。
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| Mouse Embryonic Stem Cell Maintenance for Differentiation
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Author:
Date:
2011-10-20
[Abstract] Embryonic stem cells are derived from inner cell mass of an embryo that can differentiate into every cell type in the body. Clinically, cultured red blood cell supply is of great interest. However, some of the hurdles need to be overcome. This protocol describes a protocol to maintain mouse stem cells that will be used for hematopoietic lineage differentiation assay in the lab.
[摘要] 胚胎干细胞衍生自胚胎的内细胞团，其可以分化为体内的每种细胞类型。 临床上，培养的红细胞供应是非常有趣的。 然而，一些障碍需要克服。 该协议描述了用于维持小鼠干细胞的方案，其将用于实验室中的造血谱系分化测定。
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