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Antibiotics

抗生素

Company: AppliChem
Catalog#: A1493
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Substrate Specificity of Recombinant Ser/Thr Protein Kinase
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2015-03-20
[Abstract]  Protein kinases are enzymes that phosphorylate proteins in a cell. Determination of kinase activity in reactions of phosphorylation is a very convenient way for a biochemical characterization of this group of enzymes. Here we describe a method to determine the activity of a recombinant Ser/Thr protein kinase using as a possible substrate MBP, H1, and BSA. [摘要]  蛋白激酶是磷酸化细胞中蛋白质的酶。 磷酸化反应中激酶活性的测定是这组酶的生物化学表征的非常方便的方法。 在这里,我们描述了一种使用可能的底物MBP,H1和BSA确定重组Ser/Thr蛋白激酶活性的方法。

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