| In vitro Detection of Neutrophil Traps and Post-attack Cell Wall Changes in Candida Hyphae
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Author:
Date:
2017-04-05
[Abstract] In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell wall, making them promising tools for exploring host-pathogen interactions during fungal infection.
[摘要] 在本协议中，我们描述了如何在小鼠嗜中性粒细胞与真菌白色念珠菌的真菌菌丝共培养的背景下观察嗜中性粒细胞胞外捕获物（NETs）和真菌细胞壁变化。 这些方案很容易调整，以测试与免疫细胞对真菌和细胞壁的影响有关的各种假设，使其成为探索真菌感染期间宿主病原体相互作用的有前景的工具。
C。白色念珠菌是一种多态性机会性酵母，嗜中性粒细胞是免疫细胞，对于这种和其他真菌病原体的防御至关重要（Brown等，2012; Lionakis和Netea，2013）。 NETs是可以针对病原体部署的潜在防御机制，并且已经表明它们优先针对诸如C的微生物细胞部署。白念珠菌菌丝太大而不能吞噬（Urban et al。，2006; Bruns等人，2010; Branzk等人，，2014; Rohm等人，2014）。 NET已经显示含有许多组分，包括髓过氧化物酶，胞外DNA和瓜氨酸化的组蛋白（Amulic等人，2012; ...
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| T Cell Transfer Model of Colitis
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Author:
Date:
2016-07-05
[Abstract] Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC) is mainly caused by disordered immune regulation and dysregulated intestinal microbiota. Here we present the T cell transfer model which has extensively used in many studies to identify the regulatory T cell function in gut inflammation. Naïve T cells only or together with Treg cells isolated from different donors were transferred into immunodeficient Rag1-/- mice and the disease progression was assessed by the loss of body weight and the scoring analysis. This model provides a reliable work system for the study of gut inflammation.
[摘要] 炎症性肠病（IBD），包括克罗恩病（CD）和溃疡性结肠炎（UC），主要由无序的免疫调节和肠道微生物群失调引起。 在这里我们提出的T细胞转移模型已广泛用于许多研究，以确定调节性T细胞功能的肠道炎症。 仅将来自不同供体的原始T细胞或与分离自不同供体的Treg细胞一起转移至免疫缺陷型Rag1 -/- 小鼠中，并通过体重损失和评分分析评估疾病进展。 这种模型为肠炎症的研究提供了可靠的工作系统。
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| Isolation of Lung Infiltrating Cell in Mice
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Author:
Date:
2015-02-20
[Abstract] Inflammatory lung diseases induce strong leukocyte recruitment into the organ, culminating in pneumonia area formation. Here, we describe the protocol for isolation of lung infiltrating cells. Using this assay, we analyzed the lung cell phenotyping by flow cytometry and spontaneous cytokine production by cultivating lung cells ex vivo (Amaral et al., 2014).
[摘要] 炎性肺疾病诱导强烈的白细胞募集到器官中，最终导致肺炎区域形成。 在这里，我们描述的隔离肺浸润细胞的协议。 使用该测定，我们通过流式细胞术和通过离体培养肺细胞的自发细胞因子产生来分析肺细胞表型（Amaral等人，2014）。
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