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Author:
Date:
2015-10-20
[Abstract] The bone marrow is the site of hematopoiesis and contains mixed population of blood cells including erythrocytes, granulocytes, monocytes, dendritic cells, lymphocytes and hematopoietic stem cells. The following protocol provides a simple and fast method for isolation of bone marrow immune cells (no erythrocytes) from mouse femurs with a yield of approximate 8 x 107 cells in 5 ml culture media (1.6 x 104 cells/µl). Further isolation or flow cytometric analysis might be required for study of specific immune cell types.
[摘要] 骨髓是造血的部位，包含红细胞，粒细胞，单核细胞，树突状细胞，淋巴细胞和造血干细胞等混合血细胞。 以下方案提供了一种简单而快速的方法，用于在5ml培养基（1.6×10 4个细胞/μl）中分离来自小鼠股骨的骨髓免疫细胞（无红细胞），产量约为8×10 7个细胞。 研究特异性免疫细胞类型可能需要进一步的分离或流式细胞分析。
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