| Isolation of CNS-infiltrating and Resident Microglial Cells
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Author:
Date:
2015-01-20
[Abstract] Variety of immunological and biochemical studies associated with infection or inflammation in the central nervous system (CNS) utilize CNS-resident and/or infiltrating cells which were isolated from the CNS of naïve and affected mice in order to investigate the underlying mechanisms and the potential roles of the cell populations. Mechanical and enzyme-based single cell preparations of CNS cells are subjected to a density gradient to obtain functional single cells. In combination with cell-specific biomarkers, the function and/or status of resident microglia and infiltrating lymphocytes, including B and T cells as well as macrophages, can be characterized.
[摘要] 与中枢神经系统（CNS）中的感染或炎症相关的免疫学和生物化学研究的多样性利用从初始和受影响的小鼠的CNS中分离的CNS驻留和/或浸润细胞，以研究潜在的机制和潜在的作用 的细胞群体。 将CNS细胞的机械和基于酶的单细胞制剂进行密度梯度以获得功能性单细胞。 与细胞特异性生物标志物组合，可以表征驻留小胶质细胞和浸润淋巴细胞（包括B和T细胞以及巨噬细胞）的功能和/或状态。
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| Isolation of Dendritic Cells and Macrophages from the Murine Kidneys of Lupus by Cell Sorter
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Author:
Date:
2012-04-20
[Abstract] Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of the function of these cells should lead to a better understanding of the inflammatory processes that lead to renal impairment in SLE and other renal inflammatory diseases.
[摘要] 从SLE小鼠的肾中分离和表征单核吞噬细胞的方法对于了解疾病的病理生理学是必不可少的。 这些细胞的活化与小鼠中的临床疾病的发作相关，并且这些细胞的浸润与人类的不良预后相关。这些细胞的功能的分析应导致更好地理解导致肾损伤的炎症过程 在SLE和其他肾脏炎性疾病中
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| Immunofluorescent Staining of Murine Tissue
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Author:
Date:
2011-05-05
[Abstract] Renal immune complex deposition and leukocyte infiltration are characteristic of lupus nephritis in human patients and lupus-prone mice. This protocol describes how to stain frozen sections of murine kidney to study these features using fluorescent microscopy. This protocol was developed or modified in Dr. Anne Davidson’s lab at Feinstein Institute for Medical Research.
[摘要] 肾脏免疫复合物沉积和白细胞浸润是人类患者和易患狼疮的小鼠的狼疮肾炎的特征。 这个协议描述如何染色鼠肾脏的冷冻切片，以研究这些功能使用荧光显微镜。 这个协议是在费恩斯坦医学研究所的Anne Davidson博士的实验室中开发或修改的。
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