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Lipopolysaccharides from Escherichia coli 0111:B4

来自大肠杆菌0111:B4的脂多糖

Company: Sigma-Aldrich
Catalog#: L4130
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Macrophage Inflammatory Assay
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2014-07-20
[Abstract]  Macrophages represent a widely distributed and functionally diverse population of innate myeloid cells involved in inflammatory response to pathogens, tissue homeostasis and tissue repair (Murray and Wynn, 2011). Macrophages can be broadly grouped into two subpopulations with opposing activites: M1 or pro-inflammatory macrophages that promote T-helper type 1 (Th1) cell immunity and tissue damage, and M2 or anti-inflammatory/alternatively activated macrophages implicated in Th2 response and resolution of inflammation. Here we describe a rapid assay we used previously to monitor changes in pro-inflammatory and anti-inflammatory cytokine production by lipopolysaccharide (LPS)-activated macrophages in response to therapeutic paracrine factors produced by adult stem cells (Bartosh et al. ... [摘要]  巨噬细胞代表广泛分布的和功能不同的先天骨髓细胞群,参与对病原体的炎症反应,组织内稳态和组织修复(Murray和Wynn,2011)。巨噬细胞可以大致分为具有相反活性的两个亚群:M1或促炎性巨噬细胞,其促进T辅助1型(Th1)细胞免疫和组织损伤,以及M2或抗炎或交替激活的巨噬细胞涉及Th2反应和分辨率的炎症。在这里我们描述了一种快速测定,我们以前用于监测由脂多糖(LPS)激活的巨噬细胞在响应于由成体干细胞产生的治疗性旁分泌因子的促炎和抗炎细胞因子产生中的变化(Bartosh等,/em>,2010; Ylostalo等人,2012; Bartosh 等人,2013)。该测定可以适当地适应于测试巨噬细胞对其它试剂的响应,在本文中将称为"测试试剂"或"测试化合物"。在该方案中,小鼠巨噬细胞细胞系J774A.1被扩增作为陪替氏培养皿上的粘附单层,允许容易地收获细胞而没有可以损伤细胞的酶或细胞刮擦器。然后用LPS悬浮刺激大孔,并接种到含有试验试剂的12孔细胞培养板中。 16-18小时后,收集由巨噬细胞调节的培养基,并用酶联免疫吸附测定(ELISA)测定培养基中的细胞因子谱。我们常规测量促炎细胞因子TNF-α和抗炎细胞因子白细胞介素-10(IL-10)的水平。

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