| Chikungunya ELISA Protocol
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Author:
Date:
2014-02-20
[Abstract] This protocol will result in the accurate qualitative measurement of anti-Chikungunya virus antibody (Ab) from infected mouse tissue or serum. This assay was developed by Dr. Caitlin Briggs, Arbovax, Inc. Chikungunya is a BL3 agent and should be handled in a biosafety level 3 laboratory under BL3 conditions. This protocol was used in the publication “Chikungunya virus host range E2 transmembrane deletion mutants induce protective immunity against challenge in C57BL/6J mice” (Piper et al., 2013).
[摘要] 该协议将导致从受感染的小鼠组织或血清中准确定性测量抗基孔肯雅病毒抗体（Ab）。 该测定由Dr.Citlin Briggs，Arbovax，Inc。开发。Chikungunya是BL3剂，并且应在BL3条件下在生物安全级3实验室中处理。 该方案用于出版物"Chikungunya病毒宿主范围E2跨膜缺失突变体诱导针对C57BL/6J小鼠中的攻击的保护性免疫"（Piper等人，2013）。
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| Western Blot for Detecting Phosphorylated STAT3
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Author:
Date:
2011-08-20
[Abstract] The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. STAT3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Western blot is most commonly used to detect the activation of STAT3 by using an antibody that is specific for the phosphorylated tyrosine705.
[摘要] STAT3转录因子是许多细胞因子和生长因子受体的重要信号分子，并且在许多人肿瘤中被组成型激活，并且具有致癌潜力和抗凋亡活性。 STAT3被Tyr705处的磷酸化激活，其诱导二聚化，核转位和DNA结合。 蛋白质印迹最常用于通过使用对磷酸化酪氨酸特异的抗体来检测STAT3的活化。
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