|
Author:
Date:
2014-05-05
[Abstract] Macrophage recognition of Candida albicans (C. albicans) is facilitated by pattern recognition receptors that interact with the fungal pathogen associated molecular patterns (PAMPs). Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various immune responses. This receptor is also known to be required for in vivo protection against C. albicans (Taylor et al., 2007). We recently showed that the Dectin-1 mediated protection in vivo is strain-dependent, and that C. albicans can adapt to modulate immune recognition by Dectin-1 (Marakalala et al., 2013). In vitro analysis, however, showed a Dectin-1-dependent and pro-inflammatory responses against all ...
[摘要] 通过与真菌病原体相关分子模式（PAMP）相互作用的模式识别受体促进白色念珠菌（< em>白色念珠菌）的巨噬细胞识别。 Dectin-1是已知识别真菌β-葡聚糖导致各种免疫应答诱导的主要巨噬细胞受体。也已知这种受体对于体内抗em的保护是必需的。白色念珠菌（Taylor et al。，，2007）。我们最近显示Dectin-1介导的体内保护是应变依赖性的，而且C。白色念珠菌可以适应于调节Dectin-1的免疫识别（Marakalala等人，2013）。然而，体外分析显示针对所有测试的菌株的Dectin-1依赖性和促炎症反应。该协议详细描述了本文中使用的体外分析。特别地，涉及活的荧光标记的方法。白色念珠菌，对病原体的巨噬细胞结合的定量和对真菌的酵母和菌丝形式的促炎反应。
|