Author:
Date:
2014-10-05
[Abstract] Transfer of antigen presenting cells in vivo is a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used for studies of nonconventional antigens such as CD1-presented lipids. In a recent study focusing on CD1d-restricted glycolipid antigen presentation to Natural Killer T cells, we compared antigen presenting properties of splenic B cells, CD8αPos dendritc cells (DCs) and CD8αNeg DCs (Arora et al., 2014). This protocol describes the detailed method used for isolation of these cell populations, and their transfer into recipient mice to analyze their ...
[摘要] 体内转移抗原呈递细胞是免疫学家用来检查所选择的细胞群体的抗原呈递效力的方法。该方法最常用于分析蛋白质抗原对MHC I类或II类限制性T细胞的呈递,但其也可用于非常规抗原如CD1呈递脂质的研究。在最近关于CD1d限制性糖脂抗原呈递到天然杀伤T细胞的研究中,我们比较脾B细胞,CD8αpos 树突细胞(DC)和CD8α Neg sup> DC(Arora et al。,2014)。该方案描述了用于分离这些细胞群体和将其转移到受体小鼠中以分析其抗原呈递性质的详细方法。作为总单核细胞的百分比,平均脾脏含有约1-3%骨髓树突状细胞(DC)。在绝对数量上,这转换为大约0.6-1.8×10 6个DC。为了增强脾中DC的数目,向小鼠皮下注射来自经过工程改造以表达fms相关的酪氨酸激酶3配体(Flt3L)的培养的黑素瘤细胞系(B16.Flt3L)的细胞(Mach等人。,2000)。该蛋白是与集落刺激因子-1同源的生长因子,并在造血干细胞的分化中起关键作用。将这种蛋白质作为纯化的蛋白质施用到小鼠中导致多个器官中的CD8αpos pos和CD8α阳性DC亚群的扩增。在已经植入过表达这种蛋白质的肿瘤细胞的小鼠中也观察到类似的扩增(Mach等人,2000)。根据我们的经验,来自具有可触知的B16.Flt3L肿瘤的小鼠的脾脏中高达60%的总单核细胞可以是CD11c阳性树突细胞,从而得到高达5×10 ...
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