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Author:
Date:
2015-12-20
[Abstract] Many species of cyanobacteria accumulate alkanes in their cells. It has been reported that these cyanobacteria accumulate mainly 15 long carbon chain alkane, pentadecane (C15H32), or/and 17 long chain alkane, heptadecane (C17H36). Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular pentadecane and heptadecane. We have confirmed this protocol was applicable to at least three kinds of cyanobacteria, nitrogen-fixing filamentous cyanobacterium Anabaena sp. PCC7120, non-diazotrophic unicellular cyanobacterium Synechococcus elongatus PCC7942 and halotolerant unicellular cyanobacterium Aphanothece halophytica.
[摘要] 许多种类的蓝细菌在其细胞中积累烷烃。 据报道，这些蓝细菌主要积累15个长碳链烷烃，十五烷（C 15 H 32），和/或17长链烷烃，十七烷（C 17 36）。 在这里我们描述了我们的实验室提取和定量的蓝藻细胞内十五烷和十七烷的协议。 我们已经证实该方案适用于至少三种蓝细菌，固氮的丝状蓝细菌鱼腥藻 PCC7120，非重氮营养单细胞蓝细菌细长聚球藻PCC7942和耐盐单细胞蓝细菌藻门藻。
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