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AffiniPure Goat Anti-Human IgG, Fcγ fragment specific

AffiniPure山羊抗人IgG,Fcγ片段特异性

Company: Jackson ImmunoResearch
Catalog#: 109-005-008
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Surface Plasmon Resonance Analysis of Antigen-Antibody Interaction
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2013-12-20
[Abstract]  Molecular interaction between monoclonal antibodies (MAbs) and their recognized antigen is a fundamental event leading to the neutralization activity. Estimation of their binding affinity gives beneficial information to characterize the MAbs and to develop more effective MAbs. Surface plasmon resonance (SPR) analysis is a powerful tool to analyze the molecular interaction, enabling rapid and repetitive estimation with relatively small amount of sample. Here we describe a general protocol about SPR analysis on the interaction between viral antigen and human MAb (HuMAb) IgG. Anti-human Fcγ is first covalently crosslinked on the sensor chip by amine coupling, and then HuMAb of interest is immobilized via anti-Fcγ MAb IgG interaction as ligand. Antigen injected on the sensor chip causes the ... [摘要]  单克隆抗体(MAb)与其识别的抗原之间的分子相互作用是导致中和活性的基本事件。 它们的结合亲和力的估计给出了表征MAb和产生更有效的MAb的有益信息。 表面等离子体共振(SPR)分析是分析分子相互作用的有力工具,使得能够用相对少量的样品进行快速和重复的估计。 在这里我们描述关于病毒抗原和人类MAb(HuMAb)IgG之间的相互作用的SPR分析的一般协议。 抗人Fcγ首先通过胺偶联在传感器芯片上共价交联,然后通过抗FcγMAbIgG相互作用作为配体固定目标HuMAb。 注射在传感器芯片上的抗原导致作为结合和解离的结果的时间过程中的SPR变化。 通过分析动力学,获得结合速率,解离速率和解离常数。

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