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Author:
Date:
2013-12-20
[Abstract] Use of monoclonal antibodies (MAbs) is an established laboratory strategy for characterization of specific pathogens and their antigenicity. Especially, Human MAbs (HuMAbs) with neutralizing activity against specific virus could have potential therapeutic application, and provide significant information on human epitopes that could be important for developing the next generation of universal vaccines against the virus. In addition to the classical method for murine MAb preparation, several methods for the preparation of HuMAbs have been developed. Here, we describe the development of neutralizing HuMAbs against specific virus. HuMAbs are established by fusion of the peripheral blood mononuclear cells of vaccinated volunteers or patients with the fusion partner cell line, named SPYMEG. ...
[摘要] 使用单克隆抗体（MAbs）是用于表征特定病原体及其抗原性的已建立的实验室策略。 特别地，具有针对特定病毒的中和活性的人MAb（HuMAb）可具有潜在的治疗应用，并提供对于可能对于开发下一代针对该病毒的通用疫苗重要的人表位的重要信息。 除了用于鼠MAb制备的经典方法外，已经开发了几种制备HuMAbs的方法。 在这里，我们描述中和HuMAbs针对特定病毒的发展。 HuMAbs通过将接种的志愿者或患者的外周血单核细胞与融合伴侣细胞系（称为SPYMEG）融合而建立。 然后确认每个制备的HuMAb是否可以通过体外中和测定中和特异性病毒。
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