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Collagenase type VIII

来自溶组织梭菌的胶原酶

Company: Sigma-Aldrich
Catalog#: C2139
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Isolation of Joint-infiltrating Cells
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Date:
2016-09-05
[Abstract]  Infiltration of leukocytes into joints is one of the main features of autoimmune inflammatory arthritis. Here, we describe the protocol for isolation of joint-infiltrating cells in mice. This protocol is useful to analyze cell surface antigens and intracellular cytokines by flow cytometry. [摘要]  白细胞渗入关节是自身免疫性炎症性关节炎的主要特征之一。 在这里,我们描述的协议隔离的关节浸润细胞在小鼠。 该方案可用于通过流式细胞术分析细胞表面抗原和细胞内细胞因子。

Isolation, Culture and Differentiation of Primary Acinar Epithelial Explants from Adult Murine Pancreas
Author:
Date:
2013-07-05
[Abstract]  The adult pancreas possesses an intrinsic developmental plasticity whereby acinar cells can convert into ductal structures under some pathological conditions. Acinar tissue can be isolated from murine pancreas and kept in three-dimensional collagen culture. Acinar to ductal metaplasia can be induced in primary acinar epithelial explants by treatment with growth factors. This method can be utilized in ex vivo studies involving pancreatic epithelial differentiation. [摘要]  成人胰腺具有固有的发育可塑性,其中腺泡细胞可以在一些病理条件下转化为导管结构。 腺泡组织可以从鼠胰腺中分离并保存在三维胶原培养物中。 腺泡到导管化生可以通过用生长因子治疗在原发性腺泡上皮外植体中诱导。 该方法可以用于涉及胰腺上皮分化的离体研究。

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