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Author:
Date:
2013-03-20
[Abstract] Natural killer T (NKT) cells bridge the innate and adaptive arms of the immune system, and manipulating their effector functions can have therapeutic significances in the treatment of autoimmunity, transplant biology, infectious disease and cancer. This important lymphocyte subset regulates the immune system through their potent cytokine production following the recognition of lipid antigen present in the context of the MHC class I-like CD1d molecule, in addition their ability to directly mediate cytotoxicity. Here, we describe a method of expanding mouse invariant NKT (iNKT) cell lines from mononuclear cells isolated from the thymus, spleen, or liver using bone marrow derived dendritic cells. These iNKT cell lines can be used study their co-signaling requirements, cytokine profiles ...
[摘要] 自然杀伤T（NKT）细胞桥接免疫系统的先天和适应性臂，并且操纵它们的效应子功能可以在治疗自身免疫，移植生物学，传染病和癌症中具有治疗意义。 这种重要的淋巴细胞亚群通过它们在识别存在于MHC I类CD1d分子的背景中的脂质抗原之后的有效细胞因子产生，以及它们直接介导细胞毒性的能力来调节免疫系统。 在这里，我们描述了扩增小鼠不变NKT（iNKT）细胞系从使用骨髓衍生的树突状细胞从胸腺，脾或肝分离的单核细胞的方法。 这些iNKT细胞系可用于研究其共信号要求，细胞因子谱和细胞毒性功能，这将大大增强我们对iNKT细胞生物学的知识。
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