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Rhotekin

Rhotekin

Company: EMD Millipore
Catalog#: 14-662
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RhoGTPase Activation Assay
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Date:
2012-10-05
[Abstract]  This protocol describes procedures to assay for GTP-bound (active) form of small GTPases of the Rho superfamily in human brain cancer (glioma) cell lines but can also be applied to cells or tissues of other origins. The principle of assay is based on the property of these active GTPases to interact with their specific effectors (e.g. Rhotekin for RhoA; p21-activated kinase (PAK) for Rac1 and Cdc42. In essence, Rhotekin or PAK1 are expressed in the form of GST-fusion protein to pull down the corresponding active GTPases. [摘要]  属于黄病毒科的JEV( 病毒)与CLEC5A(C型凝集素结构域家族5,成员A) C型凝集素,与DAP12信号传导蛋白相关并且在骨髓细胞上表达,与登革热病毒的程度相同。 该方案用于通过ELISA方法进行和确定纯化的JEV颗粒与人和鼠CLEC5A.Fc融合蛋白之间的相互作用。 不同的菌株和批次的纯化JEV以及CLEC5A.Fc融合蛋白的纯度可能影响吸光度的结果。 如果用户打算进行ELISA测定,则很可能需要修饰。...

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