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DMEM W/GLUTAMAX-I PYR

DMEM W / GLUTAMAX-1 PYR

Company: Thermo Fisher Scientific
Catalog#: 21885025
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Culture of Rat Olfactory Ensheathing Cells Using EasySep® Magnetic Nanoparticle Separation
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Date:
2013-04-20
[Abstract]  Olfactory ensheathing cells (OECs) can be isolated and purified from a range of postnatal day 7-day to 10-day rat olfactory bulbs. Rat OECs express the CD271/p75NTR receptor and using the “Do-It-Yourself” magnetic nanoparticle EasySep kit from STEMCELL technologies this protocol allows the selective purification of these cells in less than 50 min. Similar procedure can be used for mouse cultures. [摘要]  嗅鞘细胞(OECs)可以从出生后第7天到10天的大鼠嗅球分离和纯化。 大鼠OEC表达CD271 / p75NTR受体,并使用STEMCELL技术的“Do-It-Yourself”磁性纳米颗粒EasySep试剂盒,该方案允许在不到50分钟内对这些细胞进行选择性纯化。 类似的方法可用于小鼠培养。

Stable Interference by shRNA with pSUPER.retro Vectors and Lipofectamine
Author:
Date:
2012-08-05
[Abstract]  RNA interference is a powerful genetic approach for efficiently silencing target genes. Expression of short hairpin RNAs (shRNAs) allows analysis of the consequences of stably silencing genes. This protocol describes a method to stably integrate shRNA constructs with pSUPER.retro vectors and Lipofectamine in RKO and H1299 cells. This method can be applied to cells from other lines with modification of drug selection and cell conditions. pSUPER.retro vectors can be also transfected into packaging cells by this same method to produce retroviral supernatants. [摘要]  RNA干扰是用于有效沉默靶基因的强有力的遗传方法。 短发夹RNA(shRNA)的表达允许分析稳定沉默基因的后果。 此协议描述了稳定整合shRNA构建体与pSUPER.retro载体和Lipofectamine在RKO和H1299细胞中的方法。 这种方法可以应用于来自其他细胞系的药物选择和细胞条件的修改。 也可以通过相同的方法将pSUPER.retro载体转染到包装细胞中以产生逆转录病毒上清液。

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